3V4H
Crystal structure of a type VI secretion system effector from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.550, 148.550, 82.573 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.970 - 2.100 |
| R-factor | 0.21887 |
| Rwork | 0.216 |
| R-free | 0.26857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y12 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.570 |
| Number of reflections | 20196 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 98.7 | 87.7 |
| Redundancy | 10.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M Citric acid, 1.4 M Ammonium Sulfate, 25% sucrose, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
