3V46

Crystal Structure of Yeast Cdc73 C-Terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	53.324, 53.324, 130.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.706 - 1.549
R-factor	0.1744
Rwork	0.172
R-free	0.21040
RMSD bond length	0.007
RMSD bond angle	1.068
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.549	4.210	1.549
Rmerge	0.054	0.043	0.380
Number of reflections	28111
<I/σ(I)>	17.5
Completeness [%]	99.3	93.7	97.8
Redundancy	7.1	6.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	PEG 8000, pH 8.0, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8	293	PEG 8000, pH 8.0, VAPOR DIFFUSION, temperature 293K