3V42
Crystal structure of renal tumor suppressor protein, folliculin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.050, 99.951, 107.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.140 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET STRUCTURE OF FOLLICULIN COLLECTED AT 2.9A |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.925 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.850 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.073 | 0.648 |
| Number of reflections | 35477 | |
| <I/σ(I)> | 14.3 | 2.1 |
| Completeness [%] | 97.6 | 61.2 |
| Redundancy | 6.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 MM BIS-TRIS, PH5.5, 200 MM LISO4, 25% PEG 3350, VAPOR DIFFUSION, TEMPERATURE 293K, VAPOR DIFFUSION, HANGING DROP |
