3V2L

Structure of Anopheles gambiae odorant binding protein 20 bound to polyethylene glycol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-29
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	34.577, 38.229, 89.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 1.800
R-factor	0.1991
Rwork	0.194
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.218
Data reduction software	d*TREK (9.9.7LDz)
Data scaling software	d*TREK (9.9.7LDz)
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.230	38.230	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.082	0.077	0.429
Total number of observations		6772	6679
Number of reflections	11263
<I/σ(I)>	7.7	18.2	2.5
Completeness [%]	98.8	94.4	100
Redundancy	4.13	4	4.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	276	29% PEG 3350, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K