3V1Y
Crystal structures of glyceraldehyde-3-phosphate dehydrogenase complexes with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.171, 128.173, 77.314 |
| Unit cell angles | 90.00, 117.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.860 |
| R-factor | 0.267 |
| Rwork | 0.234 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.860 |
| Number of reflections | 108982 |
| Completeness [%] | 99.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 291 | 16% PEG 3350 (w/v), 0.1M citric acid pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
