3V0W
Crystal structure of Fab WN1 222-5 in complex with LPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.852, 101.852, 118.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.730 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.23100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 3.700 | 1.720 |
| Rmerge | 0.047 | 0.033 | 0.256 |
| Number of reflections | 65161 | ||
| <I/σ(I)> | 15.3 | ||
| Completeness [%] | 97.9 | 97.6 | 85.1 |
| Redundancy | 5.3 | 5.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.6 | 289 | Sodium acetate, Ammonium sulfate, pH 4.6, hanging drop, temperature 289K |
