3UZT
Structure of the C13.18 RNA Aptamer in Complex with G Protein-Coupled Receptor Kinase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.346, 139.719, 60.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.880 - 3.510 |
| R-factor | 0.22035 |
| Rwork | 0.216 |
| R-free | 0.31691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain A OF PDB ENTRY 1OMW |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.103 | 0.490 |
| Number of reflections | 8251 | |
| <I/σ(I)> | 28.9 | 4.7 |
| Completeness [%] | 65.5 | 14.8 |
| Redundancy | 7.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 277 | 100 mM MES pH 5.9, 100 mM NaCl and 5% PEG 3350 supplemented with 0.1 M glycine, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
