3UXW
Crystal Structures of an A-T-hook/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.143, 66.855, 50.052 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 37.760 - 2.270 |
| R-factor | 0.22266 |
| Rwork | 0.219 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
| Number of reflections | 19462 | ||
| Completeness [%] | 94.3 | 98.3 | 56.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 | MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
