3UXU

The structure of the catalytic domain of the Sulfolobus Spindle-shaped viral integrase reveals an evolutionarily conserved catalytic core and supports a mechanism of DNA cleavage in trans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.84917
Spacegroup name	P 65 2 2
Unit cell lengths	73.963, 73.963, 176.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.320 - 2.706
R-factor	0.19394
Rwork	0.193
R-free	0.21442
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.496
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.033	0.299
Number of reflections	8414
<I/σ(I)>	5.8	42.3
Completeness [%]	99.9	100
Redundancy	13.5	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20% PEG 3350, 1% Tryptone, and 0.1 M HEPES at pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	20% PEG 3350, 1% Tryptone, and 0.1 M HEPES at pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K