3UXO
Crystal Structure of Rat DNA Polymerase Beta Mutator I260Q Apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.908, 67.400, 82.456 |
| Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
| Resolution | 24.046 - 2.100 |
| R-factor | 0.2601 |
| Rwork | 0.257 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (molecular replacement) |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.590 | 3.280 | 2.190 |
| High resolution limit [Å] | 2.077 | 2.940 | 2.080 |
| Rmerge | 0.097 | 0.594 | |
| Number of reflections | 45730 | ||
| <I/σ(I)> | 6.5 | 1.3 | |
| Completeness [%] | 99.5 | 99.5 | 99.7 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | PEG 3350, MES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
