3UXN
Crystal Structure of Rat DNA Polymerase Beta, Wild Type Apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-08 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.946, 67.447, 83.357 |
| Unit cell angles | 90.00, 116.14, 90.00 |
Refinement procedure
| Resolution | 15.002 - 2.500 |
| R-factor | 0.2554 |
| Rwork | 0.250 |
| R-free | 0.31660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.002 | 3.069 | 2.567 |
| High resolution limit [Å] | 2.500 | 2.937 | 2.500 |
| Number of reflections | 22615 | ||
| Completeness [%] | 83.9 | 89 | 77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | PEG 3350, MES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
