3UXG

Crystal structure of RFXANK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2011-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97911
Spacegroup name	C 2 2 21
Unit cell lengths	43.598, 98.354, 99.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.860 - 1.850
R-factor	0.1883
Rwork	0.187
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3so8
RMSD bond length	0.017
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.16)
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.180	49.180	1.950
High resolution limit [Å]	1.850	5.850	1.850
Rmerge	0.120	0.050	0.880
Total number of observations		4124	19184
Number of reflections	18636
<I/σ(I)>	15.0071
Completeness [%]	100.0	99.08	99.97
Redundancy	7.11	6.3	7.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25% PEG3350, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25% PEG3350, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K