3UX3
Crystal Structure of Domain-Swapped Fam96a minor dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.179, 52.246, 65.893 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 34.241 - 1.800 |
| R-factor | 0.2027 |
| Rwork | 0.200 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ux2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.776 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 29996 | |
| <I/σ(I)> | 18.06 | 2.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 278 | sodium acetate, sodium malonate, DMSO, PEG3350, zinc zcetate, pH 4.7, VAPOR DIFFUSION, temperature 278K |
