3UWX

Crystal structure of UvrA-UvrB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 42 21 2
Unit cell lengths	216.823, 216.823, 116.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.065 - 4.398
R-factor	0.2906
Rwork	0.288
R-free	0.34860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.793
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.560
High resolution limit [Å]	4.398	9.460	4.400
Rmerge	0.101	0.037	0.962
Number of reflections	17635
<I/σ(I)>	6.4
Completeness [%]	96.6	97.6	82.7
Redundancy	8.7	8.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	Na/K phosphate, PEG 1000, NaCl, trehalose, pH 8.5, vapor diffusion, sitting drop, temperature 295K