3UV2

Crystal structure of the bromodomain of human nucleosome-remodeling factor subunit BPTF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-07-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.52
Spacegroup name	P 1 21 1
Unit cell lengths	27.141, 66.809, 39.259
Unit cell angles	90.00, 106.16, 90.00

Refinement procedure

Resolution	25.000 - 1.580
R-factor	0.1647
Rwork	0.162
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ensemble of 2NXB 2oo1 2oss 2ouo 2rfj 3dai
RMSD bond length	0.016
RMSD bond angle	1.671
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.1.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	20.551	1.670
High resolution limit [Å]	1.580	5.000	1.580
Rmerge	0.050	0.021	0.518
Total number of observations		2140	7074
Number of reflections	17223
<I/σ(I)>	15.9	26	1.5
Completeness [%]	93.3	97.9	79.5
Redundancy	3.7	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.10M Na/KPO4, 20.0% PEG 3350, 10.0% EtGly, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K