3UV0
Crystal structure of the drosophila MU2 FHA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.860, 99.860, 38.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.870 - 1.900 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.22700 |
| Structure solution method | MIR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 20767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M Calcium acetate, pH 7.0, 18% PEG 3350, 50mM KCl, vapor diffusion, hanging drop, temperature 293K |
