3UU8
The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' mutant reduced in solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.350, 133.470, 159.940 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 34.110 - 3.250 |
| R-factor | 0.2259 |
| Rwork | 0.226 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eam |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.110 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.116 | 0.578 |
| Number of reflections | 57137 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 97.3 | 98.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
