3UTO
Twitchin kinase region from C.elegans (Fn31-NL-kin-CRD-Ig26)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 121.700, 158.210, 60.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.400 |
| R-factor | 0.17557 |
| Rwork | 0.175 |
| R-free | 0.21326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.870 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 45313 | |
| <I/σ(I)> | 14.43 | |
| Completeness [%] | 97.2 | 96.9 |
| Redundancy | 4.86 | 4.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 20% PEG 600, 100 mM sodium citrate pH 5.5, 50 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
