3UTG
Crystal structure of Aspergillus fumigatus UDP galactopyranose mutase complexed with UDP in reduced state
Experimental procedure
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 218.176, 218.176, 320.822 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.589 - 2.250 |
R-factor | 0.1934 |
Rwork | 0.192 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ute |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.589 | 48.589 | 2.370 |
High resolution limit [Å] | 2.250 | 7.120 | 2.250 |
Rmerge | 0.017 | 0.676 | |
Total number of observations | 33545 | 151053 | |
Number of reflections | 205755 | ||
<I/σ(I)> | 17.6 | 36.3 | 1.1 |
Completeness [%] | 97.9 | 90.9 | 99.7 |
Redundancy | 5 | 5.2 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 1.5M ammonium sulfate, 0.1M sodium acetate, 5mM L-cysteine, 0.5mM THP , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |