3UTB
Crystal Structure of Nucleosome Core Particle Assembled with the 146b Alpha-Satellite Sequence (NCP146b)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.07 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.460, 109.280, 175.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.460 - 2.200 |
R-factor | 0.233 |
Rwork | 0.232 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.387 |
Data scaling software | SCALA (3.3.15) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.828 | 58.640 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.034 | 0.484 | |
Rmeas | 0.043 | 0.634 | |
Rpim | 0.021 | 0.318 | |
Total number of observations | 14458 | 48222 | |
Number of reflections | 101720 | ||
<I/σ(I)> | 13.3 | 31.1 | 2.2 |
Completeness [%] | 98.3 | 98.3 | 89.9 |
Redundancy | 5.3 | 4.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | K-cacodylate, KCl, MnCl2, pH 6.0, temperature 291K, VAPOR DIFFUSION, HANGING DROP |