3UTA

Crystal Structure of Nucleosome Core Particle Assembled with an Alpha-Satellite Sequence Containing Two TTAAA elements (NCP-TA2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	106.510, 109.910, 182.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.130 - 2.070
R-factor	0.2411
Rwork	0.240
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.418
Data scaling software	SCALA (3.3.16)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.130	106.510	2.180
High resolution limit [Å]	2.067	6.540	2.070
Rmerge		0.063	0.452
Rmeas	0.077	0.079	0.601
Rpim	0.035	0.037	0.411
Total number of observations	413096	18774	4412
Number of reflections	99095
<I/σ(I)>	12.1	20.6	1.4
Completeness [%]	75.7	99.3	15.4
Redundancy	4.2	4.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	K-cacodylate, KCl, MnCl2, pH 6.0, temperature 291K, VAPOR DIFFUSION, HANGING DROP