3UT9

Crystal Structure of Nucleosome Core Particle Assembled with a Palindromic Widom '601' Derivative (NCP-601L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Wavelength(s)	0.80
Spacegroup name	P 21 21 21
Unit cell lengths	106.494, 109.533, 174.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.550 - 2.200
R-factor	0.2572
Rwork	0.257
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.362
Data scaling software	SCALA (3.3.15)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.819	42.545	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.047	0.507
Rmeas		0.058	0.734
Rpim		0.027	0.382
Total number of observations		18685	50703
Number of reflections	104105
<I/σ(I)>	12.3	32.2	1.6
Completeness [%]	99.8	99.4	99
Redundancy	5.4	5.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	K-cacodylate, KCl, MnCl2, pH 6.0, temperature 291K, VAPOR DIFFUSION, HANGING DROP