3USU
Crystal structure of Butea monosperma seed lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.450, 78.910, 101.850 |
| Unit cell angles | 74.30, 76.65, 86.88 |
Refinement procedure
| Resolution | 69.060 - 2.460 |
| R-factor | 0.20861 |
| Rwork | 0.205 |
| R-free | 0.28722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ipv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.942 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.060 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.120 | 0.320 |
| Number of reflections | 74831 | |
| <I/σ(I)> | 5.2 | 2.3 |
| Completeness [%] | 98.5 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 298 | 25% PEG 8000, 0.2M Phosphate Buffer Saline, 5% MPD, pH 7.4, VAPOR DIFFUSION, temperature 298K |
