3USL
Crystal Structure of LeuT bound to L-selenomethionine in space group C2 from lipid bicelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.323, 90.062, 81.968 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 37.156 - 2.710 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a65 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.810 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 23553 | |
| <I/σ(I)> | 12.2 | 3.4 |
| Completeness [%] | 98.8 | 92.2 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 100 mM NaAc, 35% MPD, 10%PEG, 50 mM MgCl2, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
