3US1
Structure of p63 DNA Binding Domain in Complex with a 22 Base Pair Response Element Containing a Two Base Pair "GC" Spacer Between Half Sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320357 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.954, 101.220, 71.501 |
| Unit cell angles | 90.00, 122.78, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.800 |
| R-factor | 0.2044 |
| Rwork | 0.202 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qyn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.116 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.149 | 0.443 |
| Number of reflections | 12343 | |
| <I/σ(I)> | 8.5 | 1.8 |
| Completeness [%] | 97.5 | 95.3 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.2 M ammonium phosphate monobasic, 0.1 M Bis-Tris, pH 6.8, 14% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
