3URO

Poliovirus receptor CD155 D1D2

Replaces:  3EOW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-23
Detector	KODAK
Wavelength(s)	1.07
Spacegroup name	P 41 21 2
Unit cell lengths	84.307, 84.307, 117.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.892 - 3.501
R-factor	0.3066
Rwork	0.305
R-free	0.34140
Structure solution method	SIRAS
RMSD bond length	0.012
RMSD bond angle	2.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.500
Number of reflections	5700
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100 mM MgSO4, 6.8 M NH4NO3, 100 mM Tris buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K