3UR8
Lower-density crystal structure of potato endo-1,3-beta-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-04 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.361, 49.056, 82.618 |
| Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
| Resolution | 19.540 - 1.260 |
| R-factor | 0.14277 |
| Rwork | 0.142 |
| R-free | 0.18244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ur7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.744 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.059 | 0.467 |
| Number of reflections | 151662 | |
| <I/σ(I)> | 19.2 | 2 |
| Completeness [%] | 95.4 | 93.8 |
| Redundancy | 4.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 0.1 M sodium acetate, 0.2 M ammonium acetate, 25% PEG 4000, 15 mM glucose, streak seeding, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
