3UR0
Crystal structures of murine norovirus RNA-dependent RNA polymerase in complex with Suramin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-29 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.840, 196.510, 109.070 |
| Unit cell angles | 90.00, 114.55, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.450 |
| R-factor | 0.20245 |
| Rwork | 0.199 |
| R-free | 0.26831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.388 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 82297 | |
| Completeness [%] | 97.6 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 1.3M ammonium sulfate, 10% glycerol, 0.1M TRIS, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
