3UQZ
X-ray structure of DNA processing protein A (DprA) from Streptococcus pneumoniae
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9330 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 300.240, 300.240, 78.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.760 - 2.700 |
R-factor | 0.2206 |
Rwork | 0.219 |
R-free | 0.24510 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXS |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 47450 | |
<I/σ(I)> | 3.1 | |
Completeness [%] | 95.9 | 92.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M MES pH 6.5, 1M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M MES pH 6.5, 1M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |