3UQE

Crystal structure of the Phosphofructokinase-2 mutant Y23D from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.54
Spacegroup name	P 2 2 21
Unit cell lengths	43.838, 89.123, 175.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 2.200
R-factor	0.22
Rwork	0.219
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cqd
RMSD bond length	0.012
RMSD bond angle	0.927
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.620
High resolution limit [Å]	2.200
Number of reflections	33605
Completeness [%]	94.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.75	291	23% PEG 4000, 0.1 M SODIUM ACETATE PH 4.75, 0.2 M AMMONIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K