3UQC
Structure of the Intracellular Kinase Homology Domain of Rv3910 at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.974, 59.382, 144.031 |
| Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
| Resolution | 47.877 - 2.256 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ouk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (- Phaser) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.891 | 2.330 |
| High resolution limit [Å] | 2.256 | 2.250 |
| Number of reflections | 46032 | |
| <I/σ(I)> | 15.7 | 3 |
| Completeness [%] | 99.3 | 93.5 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.7-0.8 M succinate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
