3UQ5

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	105.570, 267.430, 111.160
Unit cell angles	90.00, 108.64, 90.00

Refinement procedure

Resolution	19.988 - 4.200
R-factor	0.2213
Rwork	0.220
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vl0
RMSD bond length	0.012
RMSD bond angle	1.638
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_897)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.988		4.310
High resolution limit [Å]	4.200	18.780	4.200
Rmerge	0.179	0.023	0.829
Number of reflections	42057	77	3139
<I/σ(I)>	16.58	82.16	4.17
Completeness [%]	98.7	15.4	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	10-12% PEG4000, 200 mM ammonium sulfate, 50 mM N-(2-acetamido)iminodiacetic acid (ADA), pH 6.5-6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K