3UOZ
Crystal Structure of OTEMO complex with FAD and NADP (form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.762, 95.179, 93.130 |
| Unit cell angles | 90.00, 101.82, 90.00 |
Refinement procedure
| Resolution | 48.607 - 2.407 |
| R-factor | 0.1989 |
| Rwork | 0.196 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uoy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.576 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 91.157 | 91.157 | 2.510 |
| High resolution limit [Å] | 2.407 | 5.210 | 2.407 |
| Rmerge | 0.105 | 0.048 | 0.452 |
| Number of reflections | 42173 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 95.8 | 95.4 | 91 |
| Redundancy | 2.6 | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 22% PEG3350, 0.1 M sodium/potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
