3UOM
Ca2+ complex of Human skeletal calsequestrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.799, 89.793, 119.161 |
| Unit cell angles | 90.13, 89.90, 60.05 |
Refinement procedure
| Resolution | 38.900 - 2.020 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1A8Y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.015 |
| Number of reflections | 195624 |
| Completeness [%] | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 278 | 50 mM Bis-tris propane, 50 mM CaCl2, 40% MPD, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
