3UO3
Jac1 co-chaperone from Saccharomyces cerevisiae, 5-182 clone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.791, 60.450, 108.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 1.850 |
| R-factor | 0.1943 |
| Rwork | 0.192 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uo2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.714 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.500 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.071 | 0.042 | 0.791 |
| Number of reflections | 34454 | ||
| <I/σ(I)> | 11 | 2.21 | |
| Completeness [%] | 99.9 | 99.2 | 99.7 |
| Redundancy | 13.5 | 13 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.085 M Tris-HCl buffer, 0.17 M sodium acetate, 25.5% PEG-4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
