3UO2

Jac1 co-chaperone from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.606, 60.548, 99.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 2.130
R-factor	0.213
Rwork	0.210
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bvo
RMSD bond length	0.016
RMSD bond angle	1.747
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.100	50.000	2.170
High resolution limit [Å]	2.130	5.780	2.130
Rmerge	0.064	0.036	0.894
Number of reflections	20799
<I/σ(I)>	12.5		4.7
Completeness [%]	99.8	96	100
Redundancy	22.9	19.7	23.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M HEPES buffer, 0.2 M ammonium acetate, 25% PEG-3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K