3UMV
Eukaryotic Class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979764 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.351, 109.362, 97.742 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 45.555 - 1.705 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1DNP 1qnf and 1IQR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.341 |
| Number of reflections | 119326 | |
| <I/σ(I)> | 23.1 | 2.87 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 35% PEG 4K, 5% saturated Urea, 200 mM immidazole malate , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
