Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.952, 121.952, 67.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.917 - 2.040 |
| R-factor | 0.1719 |
| Rwork | 0.170 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1as3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_887)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.920 | 2.060 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.050 | 0.738 |
| Number of reflections | 30856 | |
| <I/σ(I)> | 45.3 | 2 |
| Completeness [%] | 97.9 | 79 |
| Redundancy | 6.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | Protein: 10-20 mg/mL in 50 mM HEPES, pH 8.0, 1 mM EDTA, 100 microM GDP, 5 mM DTT; well solution: 1.9M ammonium sulfite, 0.1M sodium acetate, pH 6.0, VAPOR DIFFUSION, temperature 291K |
