3UMM

Formylglycinamide ribonucleotide amidotransferase from Salmonella typhimurium: Role of the ATP complexation and glutaminase domain in catalytic coupling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.817
Spacegroup name	P 65
Unit cell lengths	148.680, 148.680, 142.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.980 - 3.200
Rwork	0.196
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.590
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.980
High resolution limit [Å]	3.200	3.200
Number of reflections	29368
Redundancy		8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	2M ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K