3UMK

X-ray structure of the E2 domain of the human amyloid precursor protein (APP) in complex with copper

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.37793
Spacegroup name	P 43
Unit cell lengths	39.760, 39.760, 126.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.740 - 2.600
R-factor	0.206
Rwork	0.206
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.740	2.740
High resolution limit [Å]	2.600	2.600
Number of reflections	5926
Completeness [%]	98.0	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.1 mM HEPES, 1 M sodium acetate, 10 mM MgCl2, 50 mM CdSO4, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K