3ULL

HUMAN MITOCHONDRIAL SINGLE-STRANDED DNA BINDING PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-01
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	51.830, 51.830, 184.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.400
R-factor	0.195
Rwork	0.195
R-free	0.23700
Structure solution method	MIR
RMSD bond length	0.015 *
RMSD bond angle	1.210 *
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	PHASES
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.055	0.064
Number of reflections	9017
<I/σ(I)>	10	2.5
Completeness [%]	92.0	80.8
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	4 *	Hilgenfeld, R., (1984) FEBS Letts., 170, 143. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop		0.5-0.7 (M)
3	1	drop	phosphate	20 (mM)
4	1	drop		3 (mM)	pH7.0
5	1	reservoir	phosphate	20-60 (mM)