3ULD
High resolution structure of DNA/RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.301, 37.672, 62.378 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.19589 |
| Rwork | 0.194 |
| R-free | 0.23463 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3twh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.940 | 1.639 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.080 |
| Number of reflections | 23657 | |
| <I/σ(I)> | 16.9 | 16.9 |
| Completeness [%] | 94.1 | 94.1 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.04 M Magnesium chloride, 0.05 M Sodium cacodylate, 5% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
