3UKG
Crystal structure of Rap1/DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 140 |
Detector technology | CCD |
Collection date | 2009-11-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.595, 102.944, 116.755 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.930 - 2.950 |
R-factor | 0.1978 |
Rwork | 0.191 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ign |
RMSD bond length | 0.010 |
RMSD bond angle | 1.480 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.930 |
High resolution limit [Å] | 2.950 |
Rmerge | 0.138 |
Number of reflections | 10759 |
Completeness [%] | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100mM Tris-HCl pH 8.0, 20% (v/v) PEGmme-550, 100mM CaCl2, and 5% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 295K |