3UK9
Galactose-specific lectin from Dolichos lablab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.990, 84.130, 93.159 |
| Unit cell angles | 89.92, 76.01, 77.00 |
Refinement procedure
| Resolution | 90.170 - 3.110 |
| R-factor | 0.2537 |
| Rwork | 0.251 |
| R-free | 0.30649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.170 | 3.190 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.096 | 0.480 |
| Number of reflections | 41323 | |
| <I/σ(I)> | 11.3 | 1.9 |
| Completeness [%] | 94.3 | 93.7 |
| Redundancy | 5.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 30% PEG 6K, 100mM MES, 5% MPD , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
