3UK7
Crystal Structure of Arabidopsis thaliana DJ-1D
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97954, 0.97955, 0.98736 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.778, 75.214, 141.673 |
Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
Resolution | 45.100 - 2.050 |
R-factor | 0.17657 |
Rwork | 0.174 |
R-free | 0.22477 |
Structure solution method | MAD |
RMSD bond length | 0.024 |
RMSD bond angle | 1.953 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 70208 | |
Completeness [%] | 97.5 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.22M NaCl, 0.1M Bis-Tri, 21% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |