3UJQ
Galactose-specific lectin from Dolichos lablab in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.990, 89.990, 125.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.340 - 2.060 |
| R-factor | 0.19895 |
| Rwork | 0.197 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.110 | 2.180 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.118 | 0.307 |
| Number of reflections | 69408 | |
| <I/σ(I)> | 8.8 | 4 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 30% PEG 4K, 100mM Tris HCl, 200mM Mg-acetate , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
