3UJM
Crystal structure of the NTF2-like domain of the Drosophila melanogaster Rasputin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.200, 80.380, 39.200 |
| Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
| Resolution | 39.160 - 2.741 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q90 chain B |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.642 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.160 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.053 | 0.435 |
| Number of reflections | 6272 | |
| <I/σ(I)> | 19.8 | 3.1 |
| Completeness [%] | 97.5 | 96.6 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2 M diammonium phosphate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
