3UJ4
Crystal structure of the apo-inositol 1,4,5-trisphosphate receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.108, 77.191, 101.509 |
| Unit cell angles | 105.36, 99.98, 101.04 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.2108 |
| Rwork | 0.208 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 133458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.1 M Hepes, (NH4)2SO4), trimethylamine N-oxide, pH 8.4, vapor diffusion, hanging drop, temperature 293K |
