Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 116.840, 116.840, 117.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.480 - 3.510 |
| R-factor | 0.25894 |
| Rwork | 0.257 |
| R-free | 0.28918 |
| Structure solution method | SIRAS |
| Starting model (for MR) | Gin Homology Model of subunit A of 1ZR4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 6313 |
| <I/σ(I)> | 8.59 |
| Completeness [%] | 99.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10% Ammonium Sulfate, 20% Ethylene Glycol, 10 mM MgSO4, 100 mM MES 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10% Ammonium Sulfate, 20% Ethylene Glycol, 10 mM MgSO4, 100 mM MES 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
