3UJ2
CRYSTAL STRUCTURE OF AN ENOLASE FROM ANAEROSTIPES CACCAE (EFI TARGET EFI-502054) WITH BOUND MG AND SULFATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-15 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 282.350, 282.210, 119.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.029 - 2.000 |
R-factor | 0.1728 |
Rwork | 0.172 |
R-free | 0.23090 |
Structure solution method | 1e9i |
Starting model (for MR) | 1e9i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.045 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 202194 | |
Completeness [%] | 63.0 | 10 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | Protein (10 mM Hepes, pH 7.8, 150 mM NaCl, 10% glycerol, 5 mM DTT, 5 mM MgCl2; Reservoir (2M Ammonium Sulfate, 100 mM Citrate pH 5.5); Cryoprotection (2M Magnesium Sulfate, 100 mM Bis-Tris pH 5.3, 20% glycerol), VAPOR DIFFUSION, SITTING DROP, temperature 298K |